Biomechanical properties of bones from rats treated with sevelamer.

نویسندگان

  • Natasa Draca
  • Ana Tikvica
  • Domagoj Eljuga
  • Damir Semenski
  • Mladen Brncić
  • Slobodan Vukicević
چکیده

Sevelamer hydrochloride is used for ten years in patients on dialysis as a phosphate binder. We have previously shown that oral application of sevelamer prevents the bone loss and increases the bone volume in ovariectomized rats. In this study we further analysed the biomechanical properties of bones from rats treated with sevelamer utilizing a threepoint bending test to determine the mechanical properties of the cortical bone of the mid-shaft femur, while the indentation test was used to determine the mechanical properties of cancellous bone in the marrow cavity of the distal femoral metaphysis. Parameters analyzed included: maximum load (F(u)), stiffness (S), energy absorbed (W), toughness (T) and ultimate strength (sigma). The intrinsic properties, stress, elastic modulus and toughness were determined from measured maximum load, strains, stiffness, energy absorbed, outer and inner diameters, and calculated bone cross-sectional moment of inertia. Sevelamer was given to rats for 25 weeks with a content of 3% of sevelamer in a standard diet, starting immediately following ovariectomy (OVX). Animals were divided to the following groups: (1) Sham; (2) Sham + sevelamer 3%; (3) OVX; (4) OVX + sevelamer 3%. Our results showed that sevelamer particularly influenced the rat trabecular bone by increasing the maximum load for 26.2%, energy absorbed for 24.2% and the ultimate strength for 26.2% in sham animals treated with sevelamer 3%, as compared to sham rats. Sevelamer 3% in OVX rats also increased the maximum load for 71.4%, stiffness for 70.7%, energy absorbed for 55.9% and the ultimate strength for 71.3% as compared to OVX controls. In the three bending test sevelamer had a very little effect on preventing loss of bone strenght in the cortical bone. These results collectively suggest that sevelamer improves bone biomechanical properties, mainly affecting trabecular bone quality in both normal and ovariectomized rats.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The influence of lead on the biomechanical properties of bone tissue in rats.

INTRODUCTION AND OBJECTIVE Environmental lead (Pb) is a serious public health problem. At high levels, Pb is devastating to almost all organs. On the other hand, it is difficult to determine a safe level of exposure to Pb. More than 90% of the Pb in the adult human body and 70% in a child's body is stored in the bones. In the presented study, the effects of lead exposure on bones were studied f...

متن کامل

Phosphate Binding with Sevelamer Preserves Mechanical Competence of Bone Despite Acidosis in Advanced Experimental Renal Insufficiency

INTRODUCTION Phosphate binding with sevelamer can ameliorate detrimental histomorphometric changes of bone in chronic renal insufficiency (CRI). Here we explored the effects of sevelamer-HCl treatment on bone strength and structure in experimental CRI. METHODS Forty-eight 8-week-old rats were assigned to surgical 5/6 nephrectomy (CRI) or renal decapsulation (Sham). After 14 weeks of disease p...

متن کامل

Effect of Autogenous Omental Free Graft on the Biomechanical Properties of Fracture Healing in Dog

Objective- To evaluate greater omentum as an autogenous free graft in enhancing bone healing.Design- Prospective descriptive trial.Animals- Ten mongrel adult dogs, 3 to 4 years old and weighing 25.2 to 31 kg, assigned into two equal experimental and control groups (5dogs/group), were used.Procedures- Mid-shaft osteotomy on right radius was followed by a partial omentectomy in all dogs. A 30x30 ...

متن کامل

Effect of Low-Level He-Ne Laser Irradiation on Biomechanical Property of Wound Healing Process in Healthy and Streptozotocin Induced Diabetic Rats

Purpose: The aim of present investigation was to determine the effects of low- level He-Ne laser therapy on biomechanical property of skin wound of healthy and streptozotocin induced diabetic (STZ-D) rats. Materials and Methods: The study was performed by experimental method.36 male adult Wistar rats weighing above 250 gr. were used. Rats were divided equally into control and experimental group...

متن کامل

تأثیر هشت هفته تمرین مقاومتی بر متغیرهای منتخب بیومکانیکی استخوان ران موش های صحرایی نر مسن

objective: The purpose of this study was to determine the effect of eight weeks of strength training on some chosen biomechanical properties of femur of old male rats.  Methods: In this experimental research sixteen old male Wistar rats around Twenty months old were assigned to sedentary control group (n=8) and strength training group (n=8). Strength training group climbed from a ladder f...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Collegium antropologicum

دوره 35 2  شماره 

صفحات  -

تاریخ انتشار 2011